Project

Part:BBa_K821006:Design

Designed by: Jinlai Zhang, Dongchang Qin   Group: iGEM12_Tianjin   (2012-09-17)


KanR label from pKD3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 36
    Illegal XbaI site found at 1258
    Illegal PstI site found at 693
    Illegal PstI site found at 1280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 693
    Illegal PstI site found at 1280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 906
    Illegal BamHI site found at 1292
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 36
    Illegal XbaI site found at 1258
    Illegal PstI site found at 693
    Illegal PstI site found at 1280
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 36
    Illegal XbaI site found at 1258
    Illegal PstI site found at 693
    Illegal PstI site found at 1280
    Illegal NgoMIV site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 182
    Illegal SapI site found at 392


Design Notes

Red recombinant system


Source

KanR label from pKD3

References